dextran hydrodynamic radius

151-166. Furthermore, the influence of temperature on the intrinsic viscosity is given by the chain flexibility parameter (dln[]/dT), which gives information about the conformation of the macromolecule chain in solution [62]. The cumulative permeation of FITC-dextran 10 K was 4.82 0.32 M, in the human skin and 5.92 0.46 M in the pig skin. Chapter 2.1.4 Suspended level viscometers for transparent liquid, Ubbelohde Viscometer page 28-31. Journal Argentine Chemical Society 100 (2013) 25-34. Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. As well as being able to measure hydrodynamic radius in the Zetasizer (if you have access to one), it's also provided with a calculator that allows 1994, 95-0355. These values indicate for dextrans in dilute aqueous solution, both methods give very similar results of the intrinsic viscosity and the respective Mw. J. Phys. 2010;4(1):117. Molecular weight determination of polysaccharides. Carbohydrate Polymers, 74, 372-378. The intrinsic viscosity classical theory of random coils at conditions []0 predicts the quotient a0 = d ln[]0/d ln M to decrease from unity to 0.5 as the chain length increases. After the pore closure examination, each sample was stored in a 10% neutral buffered formalin awaiting haematoxylin/eosin (H&E) staining. Google Scholar. ; Ghosh, T.K. Table 1 shows some values of dextrans Mark-Houwink parameters, from literature consulted, where all data are based on the logarithmic plot of intrinsic viscosity [] and molecular weight Mw. Expert Opin Drug Deliv. J. Braz. Webof overall macromolecular geometry and hydrodynamic draining. Gekko, K. (1971). In vivo iontophoretic delivery of salmon calcitonin across microporated skin. These constants can be determined experimentally by measuring the intrinsic viscosity of several polymer samples for which the molecular weight has been determined by an independent method (i.e. Characterisation of insulin analogues therapeutically available to Chem. This low polydispersity realizes that the Mark-Houwink equation is forced at a given Mw regardless of the hyperbranched nature of the dextran molecule. In addition, we evaluated the regeneration of SC after microporation. (2001). Data File Dextran. The Transdermal diffusion cell system (DHC-6TD, LOGAN instrument, USA) was used to assess FITC-dextran permeability in vitro. dextran The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. This method utilizes promising strategies that have made a significant impact in transdermal delivery [6], they include: iontophoresis, microneedles, sonophoresis, radiofrequency (RF), and chemical enhancers [7,8,9,10,11]. Dextran theta temperature between 43.14 and 44.44C for all the range of molecular weights explains the deviation at 45 and 50C [36, 37]. Table 2 compares the properties of nanoparticles coated with one or other polymer. Salunkhe SS, Bhatia NM, Pokharkar VB, Thorat JD, Bhatia MS. Topical delivery of idebenone using nanostructured lipid carriers: evaluations of sun-protection and anti-oxidant effects. of different origins. WebThe micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. J Control Release. (2007). Polymolecularity and polydispersity in Molecular weight determinations. HSE and DK helped to porate the skin and to measure TEWL. A solvent at this temperature is called a theta solvent [53]. The same way, many of these authors emphasize the non-linear or hyperbranched of dextran molecular weight range from 9 to 2000kDa [3,5,8,16, 37,38]. Appl Biochem Biotechnol. 8580 Macromolecules 34, Notes - NIST The slope is a value and intercept is equal to the ln kMH value [47]. The polymer molecule assumes a tighter configuration, and the solution has a lower intrinsic viscosity. Quantitative data are presented as the meanstandard deviation, and comparisons were carried out using one-way ANOVA (Systat Software Inc., Chicago, IL, USA). WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter (d h ~ 70 nm) on order of the collagen mesh size. 2016;9706:97061E6. If there are particles in the sample, the incident laser light gets scattered in all directions. Gold MH, Biron JA. Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation. Macromolecules, 29, 2265-2268. The human and pig skin permeability of FITC-dextran (M.W. Kasaai, Mohammad R. (2012). Gillespie, T.; Hulme, M. A. The above data are not useful because it can not be compared with those obtained in this work. Dextrans in Aqueous Solution. Experimental Review on Intrinsic Although we didnt conduct assay for inflammation, we could not find any inflammation symptoms such as redness, blisters, cracked, and thickening. International Journal of Biological Macromolecules, 23, 135-141. Carbohydrate Polymers 99, 199-207. Journal of Chemical Education, 80(9), 1036-1038. We will also conduct further studies on the regeneration of human cadaver skin and evaluate the possibilities of clinical trials. These studies on Mark-Houwink parameters are usually carried out at a given temperature, obtaining a consistent result but in a very limited temperature range [68]. Google Scholar. Advances in chemistry series Washington, DC: American Chemical Society., pp. A sugar cane native dextran as an innovative functional excipient for the development of pharmaceutical tablets. B 113, 8020-8025. Palmuti Braga Vettoria, Mary Helen; Martins Franchetti, Sandra Mara; Contiero, Jonas (2012). (2008). The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. 4h after microporation, the sizes of the micropore decreased slightly. Viscosity of aqueous solutions of polysaccharides and hydrophobically modified polysaccharides: Application of Fedors equation. Deng H, Konopka CJ, Prabhu S, Sarkar S, Medina NG, Fayyaz M, Arogundade OH, Vidana Gamage HE, Shahoei SH, Nall D, Youn Y, Dobrucka IT, Audu CO, Joshi A, Melvin WJ, Gallagher KA, Selvin PR, Nelson ER, Dobrucki LW, Swanson KS, Smith AM. Mark-Houwink-Kuhn-Sakurada Exponent at the Condition. Liu Zonghua; Yanpeng Jiao; Yifei Wang; Changren Zhou; Ziyong Zhang (2008). The Mark-Houwink exponent bears the signature of a three-dimensional configuration of a polymer chain in the solvent environment. The dextran used is from Leuconostoc mesenteroides B-512F which is essentially a linear -(1-6)-linked glucose chain with however a low percentage (2-5%) of -(1-3) branches distributed along the chain4. Naik A, Kalia YN, Guy RH. Expert Rev Med Devices. CAS Keywords: dextran hydrodynamic radius 2004;25(9):155967. A few percent of R (13) glucosidic linkages provides side chains which appear to be short. In our present study, microporated skin permeated insufficient amount of 20K FITC-dextran, suggesting that enhancement of permeability was unsuccessful with macromolecules of more than 20KM.W.. Various average molecular weights (Mv, Mn, Mw, and Mz) instead of Mv were substituted in M-H equation. In addition, the permeation rate was higher in pig skin than in human skin. The parameters of Mark-Houwink for polymers can vary with the polymer solution and temperature. where (poise) is the viscosity, the (g/cm3) fluid density, and t the liquid draining time (s) subscript zero is referred to solvent, and without subscript is referred to the solution [40], and inherent viscosity is i = r -1. WebTo address this perception, in the current study, FITC-dextran of different MWs (10, 70 and 150 kDa) was used to model the transdermal delivery of low MW biotherapeutics and mAbs with MWs of approximately 150 kDa. Solvent effects on polysaccharide conformation. MeSH terms Singh TRR, Garland MJ, Cassidy CM, Migalska K, Demir YK, Ryan SAE, Woolfson D, Donnelly RF. Global hydrodynamic analysis of the molecular flexibility of galactomannans. Markiertes Dextran wird hufig per Mikroinjektion in die Zellen eingebracht. Therefore, the trend of "a" and kM-H decreases with T, where the presence of ethylene glycol or dimethylsulfoxide strongly changes the interactions of this solvent with the macromolecule. The lack of data on the uniformity of intrinsic viscosity measurements in the system water/dextran, highlights the significant influence of the solvent and temperature [74]. Nat Biotechnol. Polysaccharides-based nanoparticles as drug delivery systems. where tr is the column retention time in minutes (2 of 0.9792). Journal of Pharmaceutical and Biomedical Analysis, 41, 1260-1267. 2.It can be found from the figure that at the start-up stage of the shaft, the hydrodynamic effect (HE) is weak, so the HP is not enough to support the shaft, which The a value is higher than calculated in this work, because these calculations were performed with number average molecular weight (Mn) which rearranges the value of a and forces him to enter into unbranched or linear molecules (a > 0.5), on the other hand, if molecular weights (Mw) ware used to value would be less than 0.5. Kirkland, J. J.; Dilks, C. H.; Rementer, S. W. (1992). Our portfolio includes for example derivatives of dextran, polysucrose, inulin and more. (1999). Armstrong, J. K.; Wenby, R. B.; Meiselman, H. J.; Fisher, T. C. (2004). Dextran, Tetramethylrhodamine, 2,000,000 MW, Lysine sharing sensitive information, make sure youre on a federal This site needs JavaScript to work properly. Hindered diffusion of dextran and ficoll in microporous membranes. To confirm the extent of skin damage and regeneration after microporation, porations were performed 2, 5, and 10 times. Aqueous solutions of amphiphilic polysaccharides: Concentration and temperature effect on viscosity. US20230040324A1 AMINOACRIDINE AND AMINOPYRENE DYES Federal government websites often end in .gov or .mil. Recent Pat Drug Deliv Formul. European Biophysical Journal, 36, 85-94. 2015 Mar 17;6:155. doi: 10.3389/fmicb.2015.00155. Dependence on Concentration. Viscosity, sedimentation, and light-scattering properties of fraction of an acidhydrolyzed dextran. Journal of Polymer Science Part B: Polymer Physics, 38, 2591-2598. The genus Weissella: taxonomy, ecology and biotechnological potential. Increased TEWL values are evidence of micropores formation. (2013). Lim SB, Tingirikari JMR, Seo JS, Li L, Shim S, Seo JH, Han NS. Ionic Polymers Based on Dextran: Hydrodynamic Properties in Aqueous Solution and Solvent Mixtures. Thomas Gutberlet (Lipoteichoic Acid): Statistiques d'influence In fact, short chains do contain minor branching points. Structural and biocompatibility properties of dextran from HHS Vulnerability Disclosure, Help Conducting a comparative analysis of the data shown in Table 1 can observer that parameter data Mark-Houwink a, obtained by these authors are very similar to that obtained in this work, which affirms its validity. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. Thermo Fisher Scientific. Table 3 shows the intrinsic viscosity for the rest of the dextrans obtained by using the single-point method, measured at different temperatures. Exp Cell Res. Principles of Size Exclusion Chromatography SEC (GFC Kloster, C.; Bica C.; Rochas C.; Samios, D.; Geissler, E. (2000). Biomaterials Research Prausnitz MR, Langer R. Transdermal drug delivery. A long-chain polymer molecule in solution takes on a somewhat kinked or curled shape, intermediate between a tightly curled mass (coil) and a rigid linear configuration. Dextran standards with sequence molecular weight from 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa were utilized for the plot of calibration curve. SEC of mono-carboxymethyl cellulose (CMC) in a wide range of pH, MarkHouwink constants. 2013, Article ID 360239, pp. eCollection 2015. osmotic pressure or light scattering). Ioan, C.E. (2007). Transdermal drug delivery: overcoming the skins barrier function. Advances in Physical Chemistry, Vol. For a values from 0-0.5 a rigid sphere is predicted in a ideal solvent, from 0.5-0.8 a random coil in good solvent, and from 0.8-2 a rigid or rod like shape is expected (stiff chain). Pharmacol Pharm. A similar combination involves the intrinsic viscosity and specific volume: (a/b) is called Einstein viscosity increment, and Vs is specific volume (cm3/g). Polymers | Free Full-Text | Confinement-Induced Fractionation and We observe that this maximum radius is below Hydrodynamic The molecular weight of dextran samples can be obtained by a variety of methods including light scattering, HPLC/SEC, sedimentation, osmometry and end-group analysis. (2003). ; Hogan, M.P. All possible degrees of curling can be displayed by any molecule, but there will be an average configuration which will depend on the solvent. Bookshelf Unperturbed dimensions and the theta temperature of dextran in ethylene glycol solutions. Ibrahim O, Munavalli GS, Dover JS. The authors declare that they have no competing interests. Typical examples are the classical size-independent combinations like the Flory parameter, which that combine the intrinsic viscosity, [], and the gyration radius, Rg: These quantities have been proposed along the years, by different eminent scientists, after whom they are named. WebThe study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. Calculation of MarkHouwinkSakurada (MHS) equation viscometric constants for chitosan in any solventtemperatura system using experimental reported viscometric constants data. Carbohydrate Polymers, 38, 267-271. Synthesis and characterization of blue dextrans ; Veldhuis, T.F.J. 10% neutral buffered formalin was purchased from Hisko (Gunpo, Korea). Learn More. Weissella: An Emerging Bacterium with Promising Health Benefits. 2017 Dec 12;27(1):73-78. doi: 10.1007/s10068-017-0274-0. Ultimately they concluded that the temperature increment found to be an effective force in disturbing these interactions, mainly between polymer-polymer and polymer-solvent molecules for the used system. As intrinsic viscosity of dextrans decreases with an increase in solution temperature; chain flexibility enhances with an increase in temperature (see Table 4) showing that the intrinsic viscosity of dextran (with different molecular weights Mw from 8.8 to 200kDa) decreases linearly with an temperature increase in solution, but change to high molecular weight to hyperbranched macromolecule. Gel permeation chromatography of dextrans in parenteral solutions: Calibration procedure development and method validation. All authors read and approved the final manuscript. Dextrans can function efficiently as anterograde or retrograde tracers, The unfolding of iRFP713 in a crowded milieu - PeerJ Carbohydrate Research, 340, 811-826. Dynamics of a Polymer Solution in a Rigid Matriz. WebDextran produced from Weissella cibaria JAG8 was purified and characterized. hydrodynamic Fractional thermolysis by bipolar radiofrequency facilitates cutaneous delivery of peptide and siRNA with minor loss of barrier function. Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. The value of Mv is different from Mn, Mw, and Mz in a polydisperse polymer sample [38, 60, 61, 62]. Unable to load your collection due to an error, Unable to load your delegates due to an error. High Manufacturing Standards of Molecular Probes Dextrans. Each skin pores was captured using a microscope and the obtained images were analyzed using ImageJ software (National Institutes of Health, Bethesda, USA). The Mark-Houwink parameters is calculated molecular weights range from 8.8 to 200kDa. Biological skin substitutes for wound cover and closure. Journal of American Chemical Society, 64(11), 2716-2718. Table1 shows TEWL values measured immediately after the microporation of human and pig skins. Drug Deliv. A novel correlation for protein diffusion coefficients based on molecular weight on radius of gyration. Dextran Dextran molecular size and degree of branching as a function of sucrose concentration, pH, and temperature of reaction of. Transdermal drug delivery is an attractive alternative that addresses the limitations of oral and parenteral routes of drug administration; it enables controlled release and long-term systemic drug delivery through the skin, avoids hepatic first-pass effect, avoids gastrointestinal drug degradation, reduces discomfort and trauma resulting from hypodermic injections, and prevents safety hazards associated with dangerous medical waste from needles [1, 2]. However, considering the trauma and unethical practices associated with animal experiments, in vitro studies with human cadaver skin offers an ethical and viable alternative. Transdermal drug delivery system for non-steroidal anti inflammatory drugs: a review. Corp. (Seoul, Korea). Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. Kim, D.; Robyt, J. F.; So-Young Lee; Jin-Ha Lee; Young-Min Kim. Manage cookies/Do not sell my data we use in the preference centre. Soc., 21, 1, 78-86. Analytical Chemistry. Chem. In this sense, the largest deviations were obtained for the highest molecular weight (580-2000kDa with values from 0.8467 to 0.7346). The temperature increment can induce a macromolecule compaction (decreases in RH and []), generating by this way, a greater difficult to flowing due at requires an increase of energy consumption (Eavf<). At 25C both values of a and kM-H are within the range reported by [3, 8, 16]. (2007). After microporation, the TEWL values of the human cadaver skin increased from 11.30.6 to 33.01.0, 10.71.5 to 45.31.5, and 10.70.6 to 61.02.0g/m2/h when the microporation was performed 2, 5, and 10 times, respectively. Viet, D.; Beck-Candanedo, S.; Gray D. G. (2008). Walkenhorst, S.; Olivier, R. (2002). WebAccording to the above theoretical analysis for the micro-roughness contact model, the relationship between the hydrodynamic pressure (HP) and the contact pressure (CP) is shown in Fig. Theory, Estimation, Experiment, and Data. ACS Nano. An official website of the United States government. Progr. It is a measure of the width of molecular weight distributions (MWD) as well as probability of molecular weight distribution curve (GPC). The importance of this type of study lies in the analysis of the polysaccharide behavior at industrial processes, the requirements to reduce energy, avoid flow problems and product quality control. Sintov AC, Krymberk I, Daniel D, Hannan T, Sohn Z, Levin G. Radiofrequency-driven skin microchanneling as a new way for electrically assisted transdermal delivery of hydrophilic drugs. WebIn this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. Treatment of acne scars by fractional bipolar radiofrequency energy. Biodegradable dextran hydrogels crosslinked by stereocomplex formation for the controlled release of pharmaceutical proteins. Hydrodynamic Properties of Tragacanthin. This study demonstrated that hydrodynamic volume and radius of gyration of a dextran sample with M w < 20 kDa and its linear counterpart with equal M w are almost Careers. Radiofrequency with microneedling. The human skins were from the back or thigh of a 24-year-old woman. ; Bataille, B. Crini, G. (2005). Granath, K. A. ; Picout, D.R. Carbohydrate Polymers 88 373-381. California Privacy Statement, FOIA Carbohydrate Polymers 88 1440-1444. 2009;8(2):1739. After 24h, the cumulative permeation of FITC-dextran M.W. Table 2 compares the properties of nanoparticles coated with one or other polymer. Masuelli, Martin A. Hydrodynamic properties of whole arabic gum. Stenekes, R.J.H. Carbohydrate Polymers 87, 405-410. From Table 1, It is worth noting that the value of 2 is above 0.95, being an acceptable value for viscosimetric measurement. Table 5 shows the data of Mark-Houwink parameters determined in figure 4 from linear regressions with 2 values higher than 0.9797. Levin G, Gershonowitz A, Sacks H, Stern M, Sherman A, Rudaev S, Zivin I, Phillip M. Transdermal delivery of human growth hormone through RF-microchannels. Peptide and protein transdermal drug delivery. (2003). For data requests, please contact the authors. In a good solvent, a temperature increase results in an intrinsic viscosity decrease and in a less-extended conformation (RH<), because the entropy value increases with an increase in temperature and it is unfavorable for an extended conformation. Karmarkar, S.; Garber, R.: Kluza, J.; Koberda, M. (2006). PMC At 20C data "a" value obtained in this work is intermediate to those given by references [1, 37] while the value of kM-H is lower. Structure Properties of Dextran. GYA primarily conducted research. Hydrodynamic Viscosity of Liquids. J Cosmet Laser Ther. The Viscosity of Dilute Solutions of Long-Chain Molecules. Hydrodynamic cavitation (HC), a common phenomenon that can produce local excessive temperature, high pressure and large amount of energy. Correspondence to The optical image and histological analysis confirmed that the micropores on the skin were recovered over time. Effect of temperature on the intrinsic viscosity and conformation of chitosan in dilute HCl solution. Because of the recent animal protection rules which have restricted animal experiments [19], we used human cadaver skin as an alternative to animals. Ubbelohde, Leo. The Mark-Houwink parameters indicate the dependence with temperature (T) in the range from 20 to 50C, ie with increasing T a increases and kM-H decreases. 2006;3(3):37385. Epub 2012 May 28. Die Makromolekulare Chemie, 148(1), 229-238. dextran The range of temperatures used was 25-50 (0.1) C and it was regulated by thermostatic bath Haake C. The viscosity measurements required Hagenbach-Couette correction time (tg 37.31s). (1937). Figure5 shows the cumulative amount of the FITC-dextran (M.W. Arch Dermatol Res. Ficoll and dextran vs. globular proteins as probes for testing Human cadaver skins (fresh human full-thickness skin disc, 12~20mm) were supplied by Biopredic International (Saint-Grgoire, France). 10K, and c M.W. In this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. Durand, A. Monolayers and Thin Films of Dextran Hydrophobically Modified. 1984;17(6):11703. Sung-Wook Choi. In this study, we investigated the effectiveness of RF microporation technology in vitro, by evaluating the permeation of fluorescent-conjugated dextran (FITC-dextran) of various molecular weights through the human and pig skin after microporation, using a transdermal diffusion cell system. WebThe hydrodynamic radius ( Rh) determined by our Fluidity instruments is the Stokes radius, or the size of a hard spherical particle that diffuses at the same rate as the protein (s) detected. Radiofrequency is a well-known medical technique with expanded application in electrosurgery for laparoscopic medical procedures and in dermatology, for sebaceous glands ablation in acne treatment [12, 13], and treatment of photoaging skin symptoms, including wrinkles and hyperpigmentation [14]. The receptor chamber was filled with PBS. Table 2 shows that comparing the intrinsic viscosity data obtained from the Huggins method and the Solomon-Ciuta single-point method, a similar values are obtained and the %RE is lower for low Mw and it increases for higher Mw. Cite this article. (2013). Format), Citation-(EndNote 2. Molecular Weight Distributions of Water-Soluble Polymers by Flow Field-Flow Fractionation. European Polymer Journal, 36, 2143-2146. Where f0 is the frictional coefficient of a sphere having the same hydrodynamic (hydrated or solvated) volume V as the particle. Figure 2 shows the classical method for calculating Mark-Houwink parameters, where a linear relation between the intrinsic viscosity and the molecular weight is established for each temperature. 2021 Aug;13(4):915-925. doi: 10.1007/s12602-021-09751-1. The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. dextran hydrodynamic radius Morris, G.A. (2008). Format), Citation-(BibTeX Lee WR, Shen SC, Sun CK, Aljuffali IA, Suen SY, Lin YK, Wang JJ, Fang JY. Would you like email updates of new search results? WebTry out PMC Labs and tell us what you think. Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. Bohdanecky, Miloslav (1996). Chemical penetration enhancers for transdermal drug delivery systems. Hi Alan F Rawle sir, Can you help me in calculating the size of dextran 6 (molecular weight= 6000) and dextran 70 (molecular weight 70000)

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